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All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilution
Lane 1: human brain lysate
Lane 2: mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 66 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
P34336 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Mouse Monoclonal Antibody to GLS2
-
货号：
P34336 -
别名：
Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA -
应用：
WB,IHC -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9UI32 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to GLS2
Description |
|---|
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. |
Specification |
|
|---|---|
| Aliases | Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA |
| Entrez GeneID | 27165 |
| Swissprot | Q9UI32 |
| WB Predicted band size | 66.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This GLS2 antibody is generated from a mouse immunized with a recombinant protein of human GLS2. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
- All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- P34336 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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