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Anti-SSH3 Antibody (C-term) at 1:1000 dilution + ZR-75-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 73 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of SSH3 Antibody (C-term)(Cat#P34249) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
SSH3 Antibody (C-term) (Cat. #P34249) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SSH3 Antibody (C-term) (Cat. #P34249)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to SSH3
-
货号：
P34249 -
别名：
Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L -
应用：
WB,IHC-P,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q8TE77 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to SSH3
Description |
|---|
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]). |
Specification |
|
|---|---|
| Aliases | Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L |
| Entrez GeneID | 54961 |
| Swissprot | Q8TE77 |
| WB Predicted band size | 73.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 amino acids from the C-terminal region of human SSH3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF/ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
- Anti-SSH3 Antibody (C-term) at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Confocal immunofluorescent analysis of SSH3 Antibody (C-term)(Cat#P34249) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
- SSH3 Antibody (C-term) (Cat. #P34249) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- SSH3 Antibody (C-term) (Cat. #P34249)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34249
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