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Anti-SPDYA Antibody (Center) at 1:1000 dilution + PC-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
SPDYA Antibody (Center) (Cat. #P34222) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SPDYA Antibody (Center) (Cat. #P34222) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to SPDYA
-
货号：
P34222 -
别名：
Speedy protein A, Rapid inducer of G2/M progression in oocytes A, RINGO A, hSpy/Ringo A, Speedy-1, Spy1, SPDYA (HGNC:30613) -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q5MJ70 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to SPDYA
Description |
|---|
SPDYA (speedy homolog A (Drosophila)) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2. |
Specification |
|
|---|---|
| Aliases | Speedy protein A, Rapid inducer of G2/M progression in oocytes A, RINGO A, hSpy/Ringo A, Speedy-1, Spy1, SPDYA (HGNC:30613) |
| Entrez GeneID | 245711 |
| Swissprot | Q5MJ70 |
| WB Predicted band size | 36.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SPDYA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human SPDYA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Anti-SPDYA Antibody (Center) at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- SPDYA Antibody (Center) (Cat. #P34222) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- SPDYA Antibody (Center) (Cat. #P34222) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34222
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