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Rabbit Polyclonal Antibody to ATF4
-
货号:
P34199 -
别名:
Tax-responsive enhancer element B67; ATF4 protein -
应用:
WB -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
P18848 -
规格:
-
数量:
-+ -
说明书:
目录价¥1980

Rabbit Polyclonal Antibody to ATF4
Description |
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ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. |
Specification |
|
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Aliases | Tax-responsive enhancer element B67; ATF4 protein |
Swissprot | P18848 |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
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WB | 1/1000 |
Product Image
- Anti-ATF4 Antibody (S245) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat. #P34199). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).
- Western blot analysis of ATF4 Antibody (S245) Pab (Cat. #P34199) pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34199

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