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All lanes : Anti-GNG3 Antibody (C-Term) at 1:2000 dilution
Lane 1: human fetal brain lysate
Lane 2: mouse brain lysate
Lane 3: human brain lysate
Lane 4: mouse cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 8 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing U-87 MG cells stained with P34177(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34177, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to GNG3
-
货号：
P34177 -
别名：
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, GNG3, GNGT3 -
应用：
WB,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P63215 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to GNG3
Description |
|---|
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. |
Specification |
|
|---|---|
| Aliases | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, GNG3, GNGT3 |
| Entrez GeneID | 2785 |
| Swissprot | P63215 |
| WB Predicted band size | 8.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This GNG3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from human GNG3. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
- All lanes : Anti-GNG3 Antibody (C-Term) at 1:2000 dilution Lane 1: human fetal brain lysate Lane 2: mouse brain lysate Lane 3: human brain lysate Lane 4: mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing U-87 MG cells stained with P34177(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34177, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34177
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