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All lanes : Anti-USP21 (N-term P31) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 63 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of USP21-P31 Antibody (Cat.#P34169) in K562, Hela, 293 cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Polyclonal Antibody to USP21 (N-term P31)
-
货号：
P34169 -
别名：
Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23 -
应用：
WB,IHC -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9UK80 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to USP21 (N-term P31)
Description |
|---|
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates. |
Specification |
|
|---|---|
| Aliases | Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23 |
| Entrez GeneID | 27005 |
| Swissprot | Q9UK80 |
| WB Predicted band size | 62.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~53 amino acids from the N-terminal region of human USP21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
- All lanes : Anti-USP21 (N-term P31) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of USP21-P31 Antibody (Cat.#P34169) in K562, Hela, 293 cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34169
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