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All lanes : Anti-CREB1 Antibody (Center) at 1:2000 dilution
Lane 1: NIH/3T3 whole cell lysate
Lane 2: C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with P34088 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34088, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to CREB1
-
货号：
P34088 -
别名：
Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1 -
应用：
WB,FCM -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
P16220 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to CREB1
Description |
|---|
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. |
Specification |
|
|---|---|
| Aliases | Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1 |
| Entrez GeneID | 1385 |
| Swissprot | P16220 |
| WB Predicted band size | 35.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
- All lanes : Anti-CREB1 Antibody (Center) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing Hela cells stained with P34088 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34088, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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