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All lanes : Anti-RAB20 Antibody at 1:2000-1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with P34069(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34069, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Mouse Monoclonal Antibody to RAB20
-
货号：
P34069 -
别名：
Ras-related protein Rab-20, RAB20 -
应用：
WB,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q9NX57 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to RAB20
Description |
|---|
Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. |
Specification |
|
|---|---|
| Aliases | Ras-related protein Rab-20, RAB20 |
| Entrez GeneID | 55647 |
| Swissprot | Q9NX57 |
| WB Predicted band size | 26.3kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RAB20 antibody is generated from a mouse immunized with recombinant protein from human RAB20. |
Application |
|
|---|---|
| WB | 1/2000-1/4000 |
| FCM | 1/25 |
Product Image
- All lanes : Anti-RAB20 Antibody at 1:2000-1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing Hela cells stained with P34069(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34069, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34069
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