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Anti-POU2AF1 Antibody (N-term) at 1:2000 dilution + Ramos whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 27 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
POU2AF1 Antibody (N-term) (Cat. #P34050) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
POU2AF1 Antibody (N-term) (Cat. #P34050)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to POU2AF1 (N-term)
-
货号：
P34050 -
别名：
POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1 -
应用：
WB,IHC,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q16633 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to POU2AF1 (N-term)
Description |
|---|
POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. |
Specification |
|
|---|---|
| Aliases | POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1 |
| Entrez GeneID | 5450 |
| Swissprot | Q16633 |
| WB Predicted band size | 27.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This POU2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human POU2AF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Anti-POU2AF1 Antibody (N-term) at 1:2000 dilution + Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- POU2AF1 Antibody (N-term) (Cat. #P34050) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- POU2AF1 Antibody (N-term) (Cat. #P34050)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34050
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