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Anti-TPH1 Antibody (N-term) at 1:500 dilution + 293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of TPH1 Antibody (N-term)(Cat#P34046) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of widr cells using TPH1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue with TPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to TPH1 (N-term)
-
货号：
P34046 -
别名：
Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH -
应用：
WB,IHC-P,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P17752 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to TPH1 (N-term)
Description |
|---|
TPH1 is a member of the aromatic amino acid hydroxylase family. This protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. |
Specification |
|
|---|---|
| Aliases | Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH |
| Entrez GeneID | 7166 |
| Swissprot | P17752 |
| WB Predicted band size | 51.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human TPH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 |
| IHC | 1/100-1/500 |
| IF/ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
- Anti-TPH1 Antibody (N-term) at 1:500 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Confocal immunofluorescent analysis of TPH1 Antibody (N-term)(Cat#P34046) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Flow cytometric analysis of widr cells using TPH1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human brain tissue with TPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34046
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