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All lanes : Anti-Leo1 Antibody (N-term) at 1:1000-1:2000 dilution
Lane 1: human skeletal muscle lysate
Lane 2: mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 75 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-Leo1 Antibody (N-term) (Cat. #P34019) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.
Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term) (Cat.#P34019). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).
Rabbit Polyclonal Antibody to LEO1 (N-term)
-
货号：
P34019 -
别名：
RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL -
应用：
WB -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q8WVC0 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to LEO1 (N-term)
Description |
|---|
The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. |
Specification |
|
|---|---|
| Aliases | RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL |
| Entrez GeneID | 123169 |
| Swissprot | Q8WVC0 |
| WB Predicted band size | 75.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LEO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the N-terminal region of human LEO1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
Product Image
- All lanes : Anti-Leo1 Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of anti-Leo1 Antibody (N-term) (Cat. #P34019) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.
- Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term) (Cat.#P34019). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34019
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