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Anti-RYK Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A431 cells stained with P33893(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33893, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Mouse Monoclonal Antibody to RYK

货号：
P33893
别名：
Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A
产品上架时间：
2024-01-01
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P34925
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to RYK

Description
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

Description

May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

Specification
Aliases	Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A
Entrez GeneID	6259
Swissprot	P34925
WB Predicted band size	67.8kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This RYK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 260-565 amino acids from human RYK.