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All lanes : Anti-RPL18A Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of RPL18A Antibody (C-term) (Cat. #P33877) in Hela, 293 cell line lysates (35ug/lane). RPL18A (arrow) was detected using the purified Pab.
RPL18A Antibody (C-term) (Cat. #P33877) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RPL18A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to RPL18A
-
货号：
P33877 -
别名：
60S ribosomal protein L18a, RPL18A -
应用：
WB,IHC,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q02543 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to RPL18A
Description |
|---|
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL18A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18AE family of ribosomal proteins. It is located in the cytoplasm. |
Specification |
|
|---|---|
| Aliases | 60S ribosomal protein L18a, RPL18A |
| Entrez GeneID | 6142 |
| Swissprot | Q02543 |
| WB Predicted band size | 20.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RPL18A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-166 amino acids from the C-terminal region of human RPL18A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- All lanes : Anti-RPL18A Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of RPL18A Antibody (C-term) (Cat. #P33877) in Hela, 293 cell line lysates (35ug/lane). RPL18A (arrow) was detected using the purified Pab.
- RPL18A Antibody (C-term) (Cat. #P33877) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human brain tissue reacted with RPL18A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33877
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