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All lanes : Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of CAPZA1 antibody (N-term) (Cat.# P33866) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
CAPZA1 Antibody (N-term) (Cat. #P33866) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Polyclonal Antibody to CAPZA1 (N-term)
-
货号：
P33866 -
别名：
F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1 -
应用：
WB,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P52907 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to CAPZA1 (N-term)
Description |
|---|
CAPZA1 is a member of the F-actin capping protein alpha subunit family. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. |
Specification |
|
|---|---|
| Aliases | F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1 |
| Entrez GeneID | 829 |
| Swissprot | P52907 |
| WB Predicted band size | 32.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CAPZA1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000-1/4000 |
| IF | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
- All lanes : Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of CAPZA1 antibody (N-term) (Cat.# P33866) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
- Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
- CAPZA1 Antibody (N-term) (Cat. #P33866) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33866
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