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Anti-COG4 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 89 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of COG4 Antibody (C-term) (Cat.#P33865) in A2058 cell line lysates (35ug/lane). COG4 (arrow) was detected using the purified Pab.
Flow cytometric analysis of jurkat cells using COG4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skeletal muscle with COG4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to COG4
-
货号：
P33865 -
别名：
Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4 -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9H9E3 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to COG4
Description |
|---|
Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4. |
Specification |
|
|---|---|
| Aliases | Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4 |
| Entrez GeneID | 25839 |
| Swissprot | Q9H9E3 |
| WB Predicted band size | 89.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This COG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-785 amino acids from the C-terminal region of human COG4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Anti-COG4 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of COG4 Antibody (C-term) (Cat.#P33865) in A2058 cell line lysates (35ug/lane). COG4 (arrow) was detected using the purified Pab.
- Flow cytometric analysis of jurkat cells using COG4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human skeletal muscle with COG4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33865
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