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Anti-Fascin-3 Antibody (N-term) at 1:8000 dilution + human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of Fascin-3 Antibody (N-term) (Cat. #P33857) in MDA-MB231, NCI-H460 cell line and mouse testis tissue lysates (35ug/lane). Fascin-3 (arrow) was detected using the purified Pab.
Fascin-3 Antibody (N-term) (Cat. #P33857) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fascin-3 Antibody (N-term) (Cat. #P33857) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Fascin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to Fascin-3 (N-term)
-
货号：
P33857 -
别名：
Fascin-3, Testis fascin, FSCN3 -
应用：
WB,IHC,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q9NQT6 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to Fascin-3 (N-term)
Description |
|---|
FSCN3 acts as an actin bundling protein. |
Specification |
|
|---|---|
| Aliases | Fascin-3, Testis fascin, FSCN3 |
| Entrez GeneID | 29999 |
| Swissprot | Q9NQT6 |
| WB Predicted band size | 56.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Fascin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Fascin-3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000-1/8000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Anti-Fascin-3 Antibody (N-term) at 1:8000 dilution + human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of Fascin-3 Antibody (N-term) (Cat. #P33857) in MDA-MB231, NCI-H460 cell line and mouse testis tissue lysates (35ug/lane). Fascin-3 (arrow) was detected using the purified Pab.
- Fascin-3 Antibody (N-term) (Cat. #P33857) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Fascin-3 Antibody (N-term) (Cat. #P33857) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Fascin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33857
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