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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western blot analysis using MYLK mouse mAb against PC-3 (1), C2C12 (2), and Hela (3) cell lysate.
Immunofluorescence analysis of Hela cells using MYLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of hela cells using MYLK mouse mAb (green) and negative control (red).
Flow cytometric analysis of LNCAP cells using MYLK mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder Cancer tissues using MYLK mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using MYLK mouse mAb with DAB staining.
HOT

Mouse Monoclonal Antibody to MYLK

  • 货号:

    31895
  • 别名:

    KRP; AAT7; MLCK; MLCK1; MMIHS; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
  • 产品上架时间:

    2024-05-06
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q15746
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to MYLK

Description

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

References

1,Orphanet J Rare Dis. 2018 Mar 15;13(1):41
2,Genet Med. 2019 Jan;21(1):144-151.

Specification

Aliases KRP; AAT7; MLCK; MLCK1; MMIHS; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Entrez GeneID 4638
Swissprot Q15746
clone 1D7F6
WB Predicted band size 210kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen Purified recombinant fragment of human MYLK (AA:1375-1524) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200-1/1000
IF 1/200 - 1/1000
FCM 1/200-1/400
ELISA 1/10000