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All lanes : Anti-GARS Antibody at 1:4000 dilution
Lane 1: HT-1080 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Ramos whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling GARS with P33676 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Mouse Monoclonal Antibody to GARS
-
货号：
P33676 -
别名：
Glycine--tRNA ligase, 6.1.1.14, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS -
应用：
WB,IF -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
P41250 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to GARS
Description |
|---|
Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. |
Specification |
|
|---|---|
| Aliases | Glycine--tRNA ligase, 6.1.1.14, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS |
| Entrez GeneID | 2617 |
| Swissprot | P41250 |
| WB Predicted band size | 83.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This GARS antibody is generated from a mouse immunized with a recombinant protein between 15-305 amino acids from human GARS. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
Product Image
- All lanes : Anti-GARS Antibody at 1:4000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling GARS with P33676 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33676
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