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Anti-OPLAH Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 137 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of OPLAH Antibody (N-term) (Cat.#P33662) in K562, HepG2 cell line lysates and mouse bladder, liver tissue lysates (35ug/lane). OPLAH (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with OPLAH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to OPLAH (N-term)
-
货号：
P33662 -
别名：
5-oxoprolinase, 5-oxo-L-prolinase, 5-OPase, Pyroglutamase, OPLAH -
应用：
WB,IHC -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
O14841 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to OPLAH (N-term)
Description |
|---|
OPLAH catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate. |
Specification |
|
|---|---|
| Aliases | 5-oxoprolinase, 5-oxo-L-prolinase, 5-OPase, Pyroglutamase, OPLAH |
| Entrez GeneID | 26873 |
| Swissprot | O14841 |
| WB Predicted band size | 137.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This OPLAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human OPLAH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
- Anti-OPLAH Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of OPLAH Antibody (N-term) (Cat.#P33662) in K562, HepG2 cell line lysates and mouse bladder, liver tissue lysates (35ug/lane). OPLAH (arrow) was detected using the purified Pab.
- Formalin-fixed and paraffin-embedded human testis tissue reacted with OPLAH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33662
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