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Anti-ESPN Antibody (N-term) at 1:1000 dilution + SK-BR-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 92 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ESPN Antibody (N-term) (Cat. #P33653) in 293 cell line lysates (35ug/lane). ESPN (arrow) was detected using the purified Pab.
ESPN Antibody (N-term) (Cat. #P33653) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to ESPN (N-term)
-
货号：
P33653 -
别名：
Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein, ESPN, DFNB36 -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
B1AK53 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to ESPN (N-term)
Description |
|---|
ESPN is a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. |
Specification |
|
|---|---|
| Aliases | Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein, ESPN, DFNB36 |
| Entrez GeneID | 83715 |
| Swissprot | B1AK53 |
| WB Predicted band size | 91.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ESPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ESPN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Anti-ESPN Antibody (N-term) at 1:1000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of ESPN Antibody (N-term) (Cat. #P33653) in 293 cell line lysates (35ug/lane). ESPN (arrow) was detected using the purified Pab.
- ESPN Antibody (N-term) (Cat. #P33653) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33653
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