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Anti-ZWINT Antibody (Center) at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ZWINT antibody (Center) (Cat. #P33652) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.
Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Rabbit Polyclonal Antibody to ZWINT

  • 货号:

    P33652
  • 别名:

    ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    O95229
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to ZWINT

Description

ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.

Specification

Aliases ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT
Entrez GeneID 11130
Swissprot O95229
WB Predicted band size 31.3kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human ZWINT.
Formulation Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

Application

WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50