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Anti-TIEG2 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of TIEG2 Antibody (N-term)(Cat. #P33651) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of 293 cells using TIEG2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with TIEG2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to TIEG2 (N-term)
-
货号：
P33651 -
别名：
Krueppel-like factor 11, Transforming growth factor-beta-inducible early growth response protein 2, TGFB-inducible early growth response protein 2, TIEG-2, KLF11, FKLF, TIEG2 -
应用：
WB,IHC-P,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
O14901 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to TIEG2 (N-term)
Description |
|---|
TIEG2 is a transcription factor. The protein activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. It represses transcription of SMAD7 which enhances TGF-beta signaling. It induces apoptosis. |
Specification |
|
|---|---|
| Aliases | Krueppel-like factor 11, Transforming growth factor-beta-inducible early growth response protein 2, TGFB-inducible early growth response protein 2, TIEG-2, KLF11, FKLF, TIEG2 |
| Entrez GeneID | 8462 |
| Swissprot | O14901 |
| WB Predicted band size | 55.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TIEG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human TIEG2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF/ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
- Anti-TIEG2 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Confocal immunofluorescent analysis of TIEG2 Antibody (N-term)(Cat. #P33651) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Flow cytometric analysis of 293 cells using TIEG2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human brain tissue reacted with TIEG2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33651
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