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Anti-Meox1 (Human N-term) at 1:1000 dilution + human heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 28 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-Meox1 Antibody (N-term) (Cat. #P33577) in 293 cell line lysates (35ug/lane). Meox1(arrow) was detected using the purified Pab.
Fluorescent confocal image of Hela cell stained with Meox1 (Human N-term)(Cat#P33577).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Meox1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Meox1 immunoreactivity is localized to Nucleolus significantly.
Rabbit Polyclonal Antibody to MOX1 (Meox1) (N-term)
-
货号：
P33577 -
别名：
Homeobox protein MOX-1, Mesenchyme homeobox 1, MEOX1, MOX1 -
应用：
WB,IF -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P50221 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to MOX1 (Meox1) (N-term)
Description |
|---|
Meox1 is a member of a subfamily of non-clustered, diverged, antennapedia-like homeobox-containing genes. This protein may play a role in the molecular signaling network regulating somite development. |
Specification |
|
|---|---|
| Aliases | Homeobox protein MOX-1, Mesenchyme homeobox 1, MEOX1, MOX1 |
| Entrez GeneID | 4222 |
| Swissprot | P50221 |
| WB Predicted band size | 28.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MOX1 (Meox1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-38 amino acids from the N-terminal region of human MOX1 (Meox1). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IF | 1/10-1/50 |
Product Image
- Anti-Meox1 (Human N-term) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of anti-Meox1 Antibody (N-term) (Cat. #P33577) in 293 cell line lysates (35ug/lane). Meox1(arrow) was detected using the purified Pab.
- Fluorescent confocal image of Hela cell stained with Meox1 (Human N-term)(Cat#P33577).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Meox1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Meox1 immunoreactivity is localized to Nucleolus significantly.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33577
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