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All lanes : Anti-RAB18 Antibody at 1:500-1:2000 dilution
Lane 1: PC-3 whole cell lysate
Lane 2: mouse brain lysate
Lane 3: rat brain lysate
Lane 4: human brain lysate
Lane 5: human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 23 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing PC-3 cells stained with P33518(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33518, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Mouse Monoclonal Antibody to RAB18
-
货号：
P33518 -
别名：
Ras-related protein Rab-18, RAB18 -
应用：
WB,FCM -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9NP72 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to RAB18
Description |
|---|
Plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes. Plays a key role in eye and brain development and neurodegeneration. |
Specification |
|
|---|---|
| Aliases | Ras-related protein Rab-18, RAB18 |
| Entrez GeneID | 22931 |
| Swissprot | Q9NP72 |
| WB Predicted band size | 23.0kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB18 antibody is generated from a mouse immunized with a recombinant protein of human RAB18. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/2000 |
| FCM | 1/25 |
Product Image
- All lanes : Anti-RAB18 Antibody at 1:500-1:2000 dilution Lane 1: PC-3 whole cell lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: human brain lysate Lane 5: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing PC-3 cells stained with P33518(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33518, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33518
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