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All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: A549 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
P33495 staining PHC3 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Rabbit Polyclonal Antibody to PHC3

  • 货号:

    P33495
  • 别名:

    Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q8NDX5
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to PHC3

Description

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Specification

Aliases Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3
Entrez GeneID 80012
Swissprot Q8NDX5
WB Predicted band size 106.2kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This PHC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 819-850 amino acids from human PHC3.

Application

WB 1/2000