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All lanes : Anti-PUR4 Antibody (N-term) at 1:1000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 145 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of PUR4 Antibody (N-term) (Cat. #P33442) in HepG2 cell line lysates (35ug/lane). PUR4 (arrow) was detected using the purified Pab.
PUR4 Antibody (N-term) (Cat. #P33442) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PUR4 Antibody (N-term) (Cat. #P33442) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUR4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to PUR4 (N-term)
-
货号：
P33442 -
别名：
Phosphoribosylformylglycinamidine synthase, FGAM synthase, FGAMS, Formylglycinamide ribonucleotide amidotransferase, FGAR amidotransferase, FGAR-AT, Formylglycinamide ribotide amidotransferase, PFAS, KIAA0361 -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
O15067 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to PUR4 (N-term)
Specification |
|
|---|---|
| Aliases | Phosphoribosylformylglycinamidine synthase, FGAM synthase, FGAMS, Formylglycinamide ribonucleotide amidotransferase, FGAR amidotransferase, FGAR-AT, Formylglycinamide ribotide amidotransferase, PFAS, KIAA0361 |
| Entrez GeneID | 5198 |
| Swissprot | O15067 |
| WB Predicted band size | 144.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PUR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the N-terminal region of human PUR4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- All lanes : Anti-PUR4 Antibody (N-term) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 145 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of PUR4 Antibody (N-term) (Cat. #P33442) in HepG2 cell line lysates (35ug/lane). PUR4 (arrow) was detected using the purified Pab.
- PUR4 Antibody (N-term) (Cat. #P33442) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- PUR4 Antibody (N-term) (Cat. #P33442) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUR4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33442
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