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All lanes : Anti-ATP2A3 Antibody (N-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 114 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
P33355 staining ATP2A3 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Rabbit Polyclonal Antibody to ATP2A3 (N-Term)
-
货号：
P33355 -
别名：
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, SERCA3, SR Ca(2+)-ATPase 3, Calcium pump 3, ATP2A3 -
应用：
WB,IHC-P -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
Q93084 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to ATP2A3 (N-Term)
Description |
|---|
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. |
Specification |
|
|---|---|
| Aliases | Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, SERCA3, SR Ca(2+)-ATPase 3, Calcium pump 3, ATP2A3 |
| Entrez GeneID | 489 |
| Swissprot | Q93084 |
| WB Predicted band size | 109.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ATP2A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 13-47 amino acids from human ATP2A3. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
Product Image
- All lanes : Anti-ATP2A3 Antibody (N-Term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 114 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- P33355 staining ATP2A3 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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