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Mouse Monoclonal Antibody to PSMA5
-
货号:
P33306 -
别名:
Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5 -
应用:
WB,IHC,IF -
反应种属:
Human, Mouse, Rat -
抗体类型:
Primary antibody -
Swissprot:
P28066 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Mouse Monoclonal Antibody to PSMA5
Description |
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The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. |
Specification |
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Aliases | Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5 |
Entrez GeneID | 5686 |
Swissprot | P28066 |
WB Predicted band size | 26.4kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody. |
Formulation | Purified antibody in TBS with 0.05% sodium azide. |
Application |
|
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WB | 1/500-1/1000 |
IHC | 1/100-1/500 |
IF | 1/25 |
Product Image
- All lanes : Anti-PSMA5 Antibody at 1:1000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Neuro-2a whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- PSMA5 Antibody (Cat. #P33306) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).
- Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody(Cat#P33306). P33306 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
- Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody(Cat#P33306). P33306 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33306

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