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All lanes : Anti-PSMA5 Antibody at 1:1000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Neuro-2a whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PSMA5 Antibody (Cat. #P33306) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).

Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody(Cat#P33306). P33306 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody(Cat#P33306). P33306 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Mouse Monoclonal Antibody to PSMA5

货号：
P33306
别名：
Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
产品上架时间：
2024-01-01
应用：
WB,IHC-P,IF
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P28066
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to PSMA5

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Specification
Aliases	Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
Entrez GeneID	5686
Swissprot	P28066
WB Predicted band size	26.4kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.
Formulation	Purified antibody in TBS with 0.05% sodium azide.