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All lanes : Anti-DFFB Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: LNCaP whole cell lysates Lane 5: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of DFFB Antibody (N-term) (Cat. #P33125) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.

DFFB Antibody (N-term) (Cat. #P33125) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

DFFB Antibody (N-term) (Cat. #P33125) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Rabbit Polyclonal Antibody to DFFB (N-term)

货号：
P33125
别名：
DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
产品上架时间：
2024-01-01
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
O76075
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥1980

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Rabbit Polyclonal Antibody to DFFB (N-term)

Description
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Specification
Aliases	DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
Entrez GeneID	1677
Swissprot	O76075
WB Predicted band size	39.1kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB.
Formulation	Purified antibody in PBS with 0.05% sodium azide.