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All lanes : Anti-Suz12 Antibody (Center) at 1:2000 dilution Lane 1: mouse spleen lysates Lane 2: mouse cerebellum lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
P33066 staining (Mouse) Suz12 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Rabbit Polyclonal Antibody to (Mouse) Suz12

  • 货号:

    P33066
  • 别名:

    Polycomb protein Suz12, Suppressor of zeste 12 protein homolog, Suz12, D11Ertd530e, Kiaa0160
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q80U70
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to (Mouse) Suz12

Description

Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.

Specification

Aliases Polycomb protein Suz12, Suppressor of zeste 12 protein homolog, Suz12, D11Ertd530e, Kiaa0160
Entrez GeneID 52615
Swissprot Q80U70
WB Predicted band size 83.0kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This mouse Suz12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 381-395 amino acids from the Central region of mouse Suz12.

Application

WB 1/2000
IHC 1/100-1/500