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Anti-Bcl-G-BH3 Antibodyat 1:2000 dilution + human lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-Bcl-G BH3 Domain Pab (Cat. #P33041) in K562 cell line lysates (35ug/lane). Bcl-G BH3 Domain (arrow) was detected using the purified Pab.
Cell lysates were subjected to Western blotting to determine the expression of Bcl-gS protein. The levels of alpha-tubulin were determined to assure equal loading.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Polyclonal Antibody to Bcl-G BH3 Domain
-
货号：
P33041 -
别名：
Apoptosis facilitator Bcl-2-like protein 14, Bcl2-L-14, Apoptosis regulator Bcl-G, BCL2L14, BCLG -
应用：
WB,IHC -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9BZR8 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to Bcl-G BH3 Domain
Description |
|---|
Bcl-G belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of Bcl-G has been shown to induce apoptosis in cells. |
Specification |
|
|---|---|
| Aliases | Apoptosis facilitator Bcl-2-like protein 14, Bcl2-L-14, Apoptosis regulator Bcl-G, BCL2L14, BCLG |
| Entrez GeneID | 79370 |
| Swissprot | Q9BZR8 |
| WB Predicted band size | 36.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-229 amino acids from human Bcl. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
- Anti-Bcl-G-BH3 Antibodyat 1:2000 dilution + human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of anti-Bcl-G BH3 Domain Pab (Cat. #P33041) in K562 cell line lysates (35ug/lane). Bcl-G BH3 Domain (arrow) was detected using the purified Pab.
- Cell lysates were subjected to Western blotting to determine the expression of Bcl-gS protein. The levels of alpha-tubulin were determined to assure equal loading.
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33041
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