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Rabbit Polyclonal Antibody to SLC16A11 (N-term)
-
货号:
P33035 -
别名:
Monocarboxylate transporter 11, MCT 11, Solute carrier family 16 member 11, SLC16A11, MCT11 -
应用:
WB,IHC,FCM -
反应种属:
Human, Mouse -
抗体类型:
Primary antibody -
Swissprot:
Q8NCK7 -
规格:
-
数量:
-+ -
说明书:
目录价¥1980

Rabbit Polyclonal Antibody to SLC16A11 (N-term)
Description |
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Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates (By similarity). |
Specification |
|
---|---|
Aliases | Monocarboxylate transporter 11, MCT 11, Solute carrier family 16 member 11, SLC16A11, MCT11 |
Entrez GeneID | 162515 |
Swissprot | Q8NCK7 |
WB Predicted band size | 47.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | This SLC16A11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-76 amino acids from the N-terminal region of human SLC16A11. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
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WB | 1/500-1/1000 |
IHC | 1/100-1/500 |
FCM | 1/25 |
Product Image
- Anti-SLC16A11 Antibody (N-term)at 1:1000 dilution + mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Anti-SLC16A11 Antibody (N-term)at 1:500 dilution + A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing HT-29 cells stained with P33035 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33035, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
- Formalin-fixed and paraffin-embedded H.adrenal tissue tissue reacted with SLC16A11 Antibody (N-term) (Cat#P33035).
- P33035 staining SLC16A11 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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