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Western blot analysis of lysates from Hela cell line, mouse brain tissue lysate, HepG2 cell line (from left to right), using UBE3CAntibody(Center)(Cat. #P32988). P32988 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
UBE3C Antibody (Center) (Cat. #P32988) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE3C antibody detected the UBE3C protein (arrow).

Rabbit Polyclonal Antibody to UBE3C

  • 货号:

    P32988
  • 别名:

    Ubiquitin-protein ligase E3C, 632-, HectH2, UBE3C, KIAA0010, KIAA10
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q15386
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to UBE3C

Description

E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'-or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

Specification

Aliases Ubiquitin-protein ligase E3C, 632-, HectH2, UBE3C, KIAA0010, KIAA10
Entrez GeneID 9690
Swissprot Q15386
WB Predicted band size 123.9kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This UBE3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-578 amino acids from the Central region of human UBE3C.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000