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Western blot analysis of lysate from mouse lung tissue lysate, using Scx Antibody (N-term)(Cat. #P32967). P32967 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
P32967 staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Rabbit Polyclonal Antibody to (Mouse) Scx (N-term)

  • 货号:

    P32967
  • 别名:

    Basic helix-loop-helix transcription factor scleraxis, Scx
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q64124
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to (Mouse) Scx (N-term)

Description

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

Specification

Aliases Basic helix-loop-helix transcription factor scleraxis, Scx
Entrez GeneID 20289
Swissprot Q64124
WB Predicted band size 22.2kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.

Application

WB 1/1000
IHC 1/100-1/500