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Western blot analysis of lysate from mouse F9 cell line, using (Mouse) Tdgf1 Antibody (N-term)(Cat. #P32917). P32917 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Overlay histogram showing F9 cells stained with P32917 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P32917, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to (Mouse) Tdgf1 (N-term)
-
货号：
P32917 -
别名：
Teratocarcinoma-derived growth factor, Cripto growth factor, Epidermal growth factor-like Cripto protein, Tdgf1, Cripto -
应用：
WB,FCM -
反应种属：
Mouse -
抗体类型：
Primary antibody -
Swissprot：
P51865 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to (Mouse) Tdgf1 (N-term)
Description |
|---|
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. |
Specification |
|
|---|---|
| Aliases | Teratocarcinoma-derived growth factor, Cripto growth factor, Epidermal growth factor-like Cripto protein, Tdgf1, Cripto |
| Swissprot | P51865 |
| WB Predicted band size | 18.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Tdgf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-70 amino acids from the N-terminal region of mouse Tdgf1. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
- Western blot analysis of lysate from mouse F9 cell line, using (Mouse) Tdgf1 Antibody (N-term)(Cat. #P32917). P32917 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
- Overlay histogram showing F9 cells stained with P32917 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P32917, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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