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Western blot analysis of lysates from SH-SY5Y cell line, human brain, mouse brain tissue(from left to right), using CHRM2 Antibody(Cat. #P32873). P32873 was diluted at 1:500 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody (Cat#P32873 ). P32873 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Overlay histogram showing SH-SY5Y cells stained with (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Mouse Monoclonal Antibody to CHRM2
-
货号：
P32873 -
别名：
Muscarinic acetylcholine receptor M2, CHRM2 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P08172 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to CHRM2
Description |
|---|
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. |
Specification |
|
|---|---|
| Aliases | Muscarinic acetylcholine receptor M2, CHRM2 |
| Entrez GeneID | 1129 |
| Swissprot | P08172 |
| WB Predicted band size | 51.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This antibody is generated from a mouse immunized with a recombinant protein. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 |
| IHC | 1/100-1/500 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
- Western blot analysis of lysates from SH-SY5Y cell line, human brain, mouse brain tissue(from left to right), using CHRM2 Antibody(Cat. #P32873). P32873 was diluted at 1:500 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
- Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody (Cat#P32873 ). P32873 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
- Overlay histogram showing SH-SY5Y cells stained with (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P32873
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