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Western blot analysis of lysate from Y79 cell line, using ASMT Antibody (Center)(Cat. #P32860). P32860 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot analysis of ASMT Antibody (Center) (Cat. #P32860) in 293 cell line lysates (35ug/lane). ASMT (arrow) was detected using the purified Pab.
ASMT Antibody (Center) (Cat. #P32860) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ASMT Antibody (Center) (Cat. #P32860) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to ASMT
-
货号：
P32860 -
别名：
Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P46597 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to ASMT
Description |
|---|
ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. |
Specification |
|
|---|---|
| Aliases | Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT |
| Entrez GeneID | 438 |
| Swissprot | P46597 |
| WB Predicted band size | 38.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ASMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the Central region of human ASMT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Western blot analysis of lysate from Y79 cell line, using ASMT Antibody (Center)(Cat. #P32860). P32860 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
- Western blot analysis of ASMT Antibody (Center) (Cat. #P32860) in 293 cell line lysates (35ug/lane). ASMT (arrow) was detected using the purified Pab.
- ASMT Antibody (Center) (Cat. #P32860) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- ASMT Antibody (Center) (Cat. #P32860) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P32860
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