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Western blot analysis of PHD1 in (1) HeLa lysates; (2) A549 lysates using PHD1 antibody.
HOT
Rabbit Polyclonal Antibody to PHD1
-
货号：
P23215 -
别名：
Estrogen-induced tag 6; HPH-3; PHD1 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
Q96KS0 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to PHD1
Description |
|---|
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
Specification |
|
|---|---|
| Aliases | Estrogen-induced tag 6; HPH-3; PHD1 |
| Entrez GeneID | 112398 |
| Swissprot | Q96KS0 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PHD1/prolyl hydroxylase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/50-1/200 |
| FCM | 1/50-1/100 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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