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Western blot analysis of eIF4EBP1 in K562 lysates using 4E BP1 antibody.
Immunofluorescence analysis of 4E BP1 in HeLa using eIF4EBP1 antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using eIF4EBP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Rabbit Polyclonal Antibody to 4E BP1

  • 货号:

    P22886
  • 别名:

    EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q13541
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to 4E BP1

Description

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Specification

Aliases EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Entrez GeneID 1978
Swissprot Q13541
WB Predicted band size Calculated MW: 13 kDa; Observed MW: 18 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthesized peptide derived from human eIF4EBP1
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100
IF 1/50-1/200
FCM 1/50-1/100

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P22886