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Western blot analysis of Retinoblastoma in (1) Jurkat lysates; (2) MCF-7 lysates using Rb antibody.
Immunofluorescence analysis of Rb in SH-SY5Y using Retinoblastoma antibody.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Rabbit Polyclonal Antibody to Rb
-
货号：
P22827 -
别名：
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 -
应用：
WB,IP,IHC,IF -
反应种属：
Human,Mouse -
抗体类型：
Primary antibody -
Swissprot：
P06400 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to Rb
Description |
|---|
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo. |
Specification |
|
|---|---|
| Aliases | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Entrez GeneID | 5925 |
| Swissprot | P06400 |
| WB Predicted band size | Calculated MW: 106 kDa; Observed MW: 106 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Retinoblastoma |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IP | 1/10-1/20 |
| IHC | 1/50-1/100 |
| IF/ICC | 1/50-1/200 |
Product Image
- Western blot analysis of Retinoblastoma in (1) Jurkat lysates; (2) MCF-7 lysates using Rb antibody.
- Immunofluorescence analysis of Rb in SH-SY5Y using Retinoblastoma antibody.
- Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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