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Western blot analysis of Retinoblastoma in (1) Jurkat lysates; (2) MCF-7 lysates using Rb antibody.
Immunofluorescence analysis of Rb in SH-SY5Y using Retinoblastoma antibody.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Rabbit Polyclonal Antibody to Rb

  • 货号:

    P22827
  • 别名:

    RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC,IF
  • 反应种属:

    Human,Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P06400
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to Rb

Description

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Specification

Aliases RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Entrez GeneID 5925
Swissprot P06400
WB Predicted band size Calculated MW: 106 kDa; Observed MW: 106 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen A synthesized peptide derived from human Retinoblastoma
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100
IF 1/50-1/200