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Western blot analysis of eIF4EBP1 in mouse heart lysates using 4E BP1 antibody.
Western blot analysis of eIF4EBP1 in Jurkat, C6, CHO-K1 lysates using eIF4EBP1 antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF4EBP1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Rabbit Monoclonal Antibody to 4E BP1

  • 货号:

    P22701
  • 别名:

    EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC
  • 反应种属:

    Human,Mouse,Rat,Hamster
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q13541
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to 4E BP1

Description

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Specification

Aliases EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Entrez GeneID 1978
Swissprot Q13541
WB Predicted band size Calculated MW: 13 kDa; Observed MW: 15-20 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Hamster
Immunogen A synthetic peptide of human eIF4EBP1
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100