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Western blot analysis of UBE2I/UBC9 in 3T3, PC-12, MEF lysates using UBE2I/UBC9 antibody.
Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody
Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using SUMO Conjugating Enzyme UBC9 antibody,and DAPI(blue).
Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Rabbit Monoclonal Antibody to SUMO Conjugating Enzyme UBC9

  • 货号:

    P22621
  • 别名:

    UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC,IF
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P63279
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to SUMO Conjugating Enzyme UBC9

Description

The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain.

Specification

Aliases UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
Entrez GeneID 7329
Swissprot P63279
WB Predicted band size Calculated MW: 18 kDa; Observed MW: 18 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthetic peptide of human UBE2I/UBC9
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100
IF 1/50-1/200