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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ATG10 mouse mAb against K562 (1) cell lysate.
Flow cytometric analysis of Hela cells using ATG10 mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to ATG10
-
货号：
31729 -
别名：
APG10; APG10L; pp12616 -
应用：
WB,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9H0Y0 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to ATG10
Description |
|---|
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]). |
References |
|---|
| 1.Int J Cancer. 2016 Oct 1;139(7):1564-73. 2.PLoS One. 2012;7(12):e52705. |
Specification |
|
|---|---|
| Aliases | APG10; APG10L; pp12616 |
| Entrez GeneID | 83734 |
| Swissprot | Q9H0Y0 |
| clone | 8A9B3 |
| WB Predicted band size | 25.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATG10 (AA: 1-125) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31729
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