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Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody.
Immunocytochemistry analysis of Rad21 (green) in Hela using Rad21 antibody,and DAPI(blue).
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Rad21 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Rabbit Monoclonal Antibody to Rad21

  • 货号:

    P22549
  • 别名:

    RAD21; HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human,Hamster
  • 抗体类型:

    Primary antibody
  • Swissprot:

    O60216
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to Rad21

Description

Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspaselike enzyme, allows chromosomal separation. Rad21 interacts with Rec8 to form a cohesin complex that functions in sister chromatid alignment. Rad21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis.

Specification

Aliases RAD21; HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog
Entrez GeneID 5885
Swissprot O60216
WB Predicted band size Calculated MW: 72 kDa; Observed MW: 130 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Hamster
Immunogen A synthetic peptide of human Rad21
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/50-1/200