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Western blot analysis of BubR1 in Hela, A549, MCF-7, U251 lysates using BUBR1 antibody.
Immunocytochemistry analysis of BubR1 (green) in hela using BubR1 antibody,and DAPI(blue)
HOT
Rabbit Monoclonal Antibody to BUBR1
-
货号：
P22277 -
别名：
BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1 -
应用：
WB,IP,IF -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
O60566 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to BUBR1
Description |
|---|
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. |
Specification |
|
|---|---|
| Aliases | BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1 |
| Entrez GeneID | 701 |
| Swissprot | O60566 |
| WB Predicted band size | Calculated MW: 120 kDa; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human BubR1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IP | 1/10-1/20 |
| IF | 1/50-1/200 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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