Rabbit Monoclonal Antibody to NCE2

Description
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Specification
Aliases	NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2
Entrez GeneID	140739
Swissprot	Q969M7
WB Predicted band size	Calculated MW: 21 kDa; Observed MW: 21 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Recombinant protein of human NCE2
Formulation	Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application
WB	1/500-1/1000
IHC	1/50-1/100
IF/ICC	1/50-1/200