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Western blot analysis of LC3A in 3T3, mouse heart, mouse brain lysates using LC3A antibody.
Western blot analysis of LC3A in K562, rat Brain, Hela lysates using LC3A antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Rabbit Monoclonal Antibody to LC3A

  • 货号:

    P21638
  • 别名:

    Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9H492
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to LC3A

Description

Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

Specification

Aliases Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
Entrez GeneID 84557
Swissprot Q9H492
WB Predicted band size Calculated MW: 14 kDa; Observed MW: 14,16 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthetic peptide of human MAP1LC3A
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100