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Western blot analysis of Vimentin in Molt4, K562, COS7, Jurkat, Hela and Vimentin negative (Daudi, Ramos, Raji) lysates using Vimentin antibody.
Immunoprecipitation analysis of Vimentin in Hela lysates using Vimentin antibody.
Immunocytochemistry analysis of Vimentin in Hela using Vimentin antibody.
Immunohistochemistry analysis of paraffin-embedded Human tonsils using Vimentin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HOT
Mouse Monoclonal Antibody to Vimentin
-
货号：
P20777 -
别名：
VIM; Vimentin -
应用：
WB,IP,IHC,IF -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P08670 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to Vimentin
Description |
|---|
Vimentin an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. |
Specification |
|
|---|---|
| Aliases | VIM; Vimentin |
| Entrez GeneID | 7431 |
| Swissprot | P08670 |
| clone | 7H8 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 57 kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant human Vimentin protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IP | 1/10-1/20 |
| IHC | 1/50-1/100 |
| IF | 1/50-1/200 |
Product Image
- Western blot analysis of Vimentin in Molt4, K562, COS7, Jurkat, Hela and Vimentin negative (Daudi, Ramos, Raji) lysates using Vimentin antibody.
- Immunoprecipitation analysis of Vimentin in Hela lysates using Vimentin antibody.
- Immunocytochemistry analysis of Vimentin in Hela using Vimentin antibody.
- Immunohistochemistry analysis of paraffin-embedded Human tonsils using Vimentin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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