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Western blot analysis of DNAPKcs in Hela and K562 lysates using DNAPKcs antibody.
Western blot analysis of DNAPKcs in Hela, Molt4, A549 and HCT116 lysates using DNAPKcs antibody.
Immunoprecipitation analysis of DNA PKcs in Hela lysates using DNAPKcs antibody.
Immunocytochemistry analysis of DNA PKcs in Hela using DNAPKcs antibody.
Immunohistochemistry analysis of paraffin-embedded Breast cancer using DNAPKcs antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HOT

Mouse Monoclonal Antibody to DNA PKcs

  • 货号:

    P20726
  • 别名:

    PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC,IF
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P78527
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to DNA PKcs

Description

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Specification

Aliases PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
Entrez GeneID 5591
Swissprot P78527
clone 7G3
WB Predicted band size Calculated MW: 469 kDa; Observed MW: 450 kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
Formulation Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100
IF 1/50-1/200