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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using CD106 mouse mAb against EC (1), COS7 (2), MCF-7 (3), HepG2 (4), and Hela (5) cell lysate.
Immunofluorescence analysis of Hela cells using CD106 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HL-60 cells using CD106 mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to CD106
-
货号：
31490 -
别名：
VCAM1; INCAM-100 -
应用：
WB,IF,FCM -
反应种属：
Human,Rat,Monkey -
抗体类型：
Primary antibody -
Swissprot：
P19320 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to CD106
Description |
|---|
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. |
References |
|---|
| 1.Stem Cell Res Ther. 2016 Apr 4;7:49.2.Cell Signal. 2015 Dec;27(12):2467-73. |
Specification |
|
|---|---|
| Aliases | VCAM1; INCAM-100 |
| Entrez GeneID | 7412 |
| Swissprot | P19320 |
| clone | 6A11D8 |
| WB Predicted band size | 81.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human CD106 (AA: extra 25-183) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using CD106 mouse mAb against EC (1), COS7 (2), MCF-7 (3), HepG2 (4), and Hela (5) cell lysate.
- Immunofluorescence analysis of Hela cells using CD106 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of HL-60 cells using CD106 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31490
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